Signalling

Part:BBa_K176028:Design

Designed by: Danqian Liu, Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-07-20)

TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

Input: AHL

Output: PoPS

Design:

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates PoPS output, activated by LuxR and AHL, repressed by TetR and no aTc


Source

K176025 K176000

References