Signalling
Part:BBa_K176028:Design
Designed by: Danqian Liu, Chao Li, Hao Jiang Group: iGEM09_USTC (2009-07-20)
TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Design Notes
Input: AHL
Output: PoPS
Design:
pCon 0.70(J23101) generates LuxR
pLux/Tet(K176000) generates PoPS output, activated by LuxR and AHL, repressed by TetR and no aTc
Source
K176025 K176000